The combined filtrate is evaporated in awater bath at 67–68 °C (n-hexane boiling point is 68.742 °C), then the bulb is dried in a drying
box at 80 °C to a constantweight, cooled in a desiccator andweighed on an analytical balance to the fourth decimal digit. A constantmass
is considered achieved if the weight reduction in the last two balance measurements does not exceed 0.0005 g. The fat sample weight is
determined as the weight of the empty flask minus the weight of the flask with the resulting fat