2.4.2.2. Disk diffusion method. Sterile filter paper disks (7 cm
diameter) soaked in 100, 200, 400 or 800 mL L1 pure essential oil
was placed on the inner surface of the Petri dish lid. The dishes
were sealed with parafilm and incubated upside-down at 25 C.
Colony radius (in mm) was measured at 1e6 days. Control consists
of sterile distilled water-soaked filter paper in the lid of Petri dish.
In this treatment, all the experiments were done in four
replications.
The percentage inhibition of fungal growth was determined on
the growth in test plates compared to the respective control plates
according to the equation: