In order to extract phenotypic measurements from the images,the plant had to be separated from the background. This sep-aration was carried out either by converting the image colourspace to HSI (hue, saturation and intensity) or using the intensitychannel,intensitychannel, if the image contained a sufficient contrast between theplant and the background. Otherwise a mathematical formula,involving the three channels of the RGB colour space, was usedto calculate a new grey-scale image with sufficient contrast. Thisgrey-scale image was then subjected to thresholding to create abinary mask to extract the plant from the image. The one excep-tion was for the NIR top view images, where the mask from the RGBtop view plant image was resized and transposed to the NIR image.The measurement of digital biomass, correlated with fresh weight,was carried out as described by Eberius and Lima-Guerra (2009).Specifically, the areas of the plant in the three orthogonal imagesfrom the RGB chamber were applied in the following formula: