On the other hand, the 6-hour coloration period was effective to assess the seeds viability of this species at concentrations of 0.075%, 0.1% and 0.5%, for they provided a clear coloration of the embryos, facilitating the analysis and interpretation of results (Figures 2A, 2B, 2C and 2D). Furthermore, it allowed the identification of lesions in areas that are critical for the embryo, such as the hypocotyl-radicle axis. It should be noted, however, that the 6-hour coloration period, associated with a concentration of 1.0% tetrazolium solution (Figure 2E) provided more than 60% of embryos with an intense red color (deteriorated seeds – unviable), different from the results obtained in the germination test (Table 2).