this is a species of Thai’s native and found that in the rainforest or mixed deciduous forest, which is important in early development of Thai orchids from Thailand to the world: with the characteristic bouquet of beautiful flowers cylindrical prominent curve, each bouquet has more than 50 flowers, petal are white with purple points, has strong aroma when the bouquet of flowers are full bloom and flowers bloom in mid-winter period that is December to February (Udorn et al., 2006). Thus, the study was aimed to compare the potentiality of modified media and Vacin a nd Went media for in vitro culture of R. gigantean.