However, minimal
proliferation
was observed among all isolates (data not shown; similar
trends shown in Fig. 4). Carbon dioxide production and package
bloating occurred under these same conditions, independent of yeastlike
proliferation. Therefore, “days until visible container bloating”
was reported in Table 1 and was performed, as described in
Section 2.2, by inoculation into a sealed yogurt cup instead of in an anaerobic
chamber jar. Individual packaging of yogurt samples allowed for
headspace analysis and package destruction at every sampling point in
the challenge study.