The effect of NaCl concentrations on the antibacterial activity of
EO water generated was determined with 4 replications. All trials
for challenge studies were performed 6 times. Data were analyzed
by the general linear model procedure (PROC GLM) of SAS version
9.1 (SAS Inst. Inc., Cary, N.C., U.S.A.). Analysis of variance (ANOVA)
among treatments was performed and comparisons of mean values
were established by Tukey’s studentized range (HSD) multiple comparison test at the significant level of 0.05. When microbial colonies
were not detected on plates, a value of 1.00 was assigned to the log10 CFU/g value for the purpose of statistical analysis (Hao and others
1998)