J. curcas fruits were collected 8 weeks after the formation of pinhead sized fruits from the Universiti Putra Malaysia farm. The collected fruits were washed with distilled water to remove dust particles. The fruits were air dried on a plastic tray at ambient temperature for 3 days, then dried in an oven (Memmert, ULM 500, Germany) at 50°C for 5 to 7 days to remove residual moisture. Final moisture content of the samples was 12% (MF-50, Moisture Analyzer, US). The plant materials were extracted according to the methods of Okoh et al. (2009) and Obafemi et al. (2006) with some modifications. Pulp and seeds were separated from the whole fruits manually using a knife. The whole fruit, pulp and seeds were grinded separately by Polymix grinder