Maize leaves inoculated with B. maydis alone became yellow
with many blight spots (Fig. 2) and disease severity increased with
time. In contrast, when both B. maydis and CHM1 culture filtrate
were inoculated together, the disease severity was less, most leaves
were green with few blight spots and the disease increased little
with time. The disease index for inoculating B. maydis alone was
78.33%, but only 23.33% for CHM1 and B. maydis together, suggesting
a 70.22% protection effect (Table 2) with the addition of
CHM1.
When inoculating mycelia of R. solani on horsebean leaves,
many speckles formed quickly on the leaves (Fig. 3). After 5 days,
the entire leaves became necrotic, brown and died. However, when
spraying the CHM1 culture filtrate with R. solani on the leaves, the
leaves remained green and healthy, indicating that CHM1 has
the capacity for protecting horsebean against infection by R. solani.
The disease index for R. solani alone was 71.67% but it decreased to
26.67% when CHM1 and R. solani were inoculated together with
62.79% protection effect (Table 2).