VCO used for this study was purchased from local manufacturer
Organic Gain Sdn Bhd (Bangi, Selangor, Malaysia). It was used either in a fresh form, heated once, five times or
ten times as per earlier protocol described by Owu et al.
with few modifications.17 Briefly, 2.5 L of oil was heated
to 180 C in a stainless steel wok and used to deep-fry
1 kg sliced sweet potatoes. The heating process lasted for
15 min. The hot oil was then left to cool at room temperature
for 5 h. This procedure resulted in the once heated
VCO group (1HVCO). The pre-cooled hot oil was used to
deep-fry another new batch of sweet potatoes. The frying
process was carried out without adding any fresh oil to compensate
for oil losses. In order to obtain VCO heated five
times (5HVCO) and VCO heated ten times (10HVCO), the
same heating procedure was repeated four and nine times,
respectively. The experimental diets were prepared twice in
a week. Standard rat chow (Gold Coin, Port Klang, Selangor,
Malaysia) was ground and mixed with some water and
fresh or the heated VCO prepared. The weight ratio of rat
chow to the oil was 100:15. The mixture was then dried at
70 C overnight in an oven.
VCO used for this study was purchased from local manufacturerOrganic Gain Sdn Bhd (Bangi, Selangor, Malaysia). It was used either in a fresh form, heated once, five times orten times as per earlier protocol described by Owu et al.with few modifications.17 Briefly, 2.5 L of oil was heatedto 180 C in a stainless steel wok and used to deep-fry1 kg sliced sweet potatoes. The heating process lasted for15 min. The hot oil was then left to cool at room temperaturefor 5 h. This procedure resulted in the once heatedVCO group (1HVCO). The pre-cooled hot oil was used todeep-fry another new batch of sweet potatoes. The fryingprocess was carried out without adding any fresh oil to compensatefor oil losses. In order to obtain VCO heated fivetimes (5HVCO) and VCO heated ten times (10HVCO), thesame heating procedure was repeated four and nine times,respectively. The experimental diets were prepared twice ina week. Standard rat chow (Gold Coin, Port Klang, Selangor,Malaysia) was ground and mixed with some water andfresh or the heated VCO prepared. The weight ratio of ratchow to the oil was 100:15. The mixture was then dried at70 C overnight in an oven.
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