The data were analysed and processed using the installed Empower 2 software.
The column was maintainedat room temperature and the flow rate of the mobile phase was kept at 1.0 mL/min, which consists of methanol +acetonitrile:
phosphate buffer. Sample volume of 5000-lL was kept with the help of a Rheodyne 77251 injector and compounds were detected between 190 and 400 nm with 2996 Photo Diode Array Detector.