This would
explain the observed increase in the number of incorrect and No
Calls assigned for the heterozygous sample. Therefore, in order to
minimise call error rate, it is important to maintain the allelic
balance. Loss of heterozygosity (LOH), which is a common form of
allelic imbalance, has been used to identify genomic regions that
harbor tumor suppressor genes and to characterize tumor stages
and progression[15]. To avoid misinterpretation of such data, it is
critical that sufficient copies of the heterozygous locus are present
in the genotyping assay.