Extraction of sugars from mango peel
Mango powder (100 g) was mixed with water (1:3) and left overnight. The liquid containing sugars was extracted with the help of
cheesecloth by squeezing. This acted as control. In the case of
enzymatic digestion with 1% (v/v) pectinase, Trizyme 50 (Triton
Chemicals, Mysore, India) was used for improved results. The
extraction medium pH was 5.0 and the temperature was 37°C. The
extract was suitably diluted to obtain the desired concentration of
sugars (15-17%, w/v), and was supplemented with various nutrients
(yeast extract 1%, peptone 1.5%, ammonium phosphate 2% and
wheat bran 3%) in order to study the effect of nutrients on fermentation. The unsupplemented medium acted as the control. In the
case of wheat bran extract supplementation experiments, mango
peel sugars were extracted into the wheat bran extract solution
instead of water.