Based on the preliminary results, a media composition was standardized for A. racemosus. The details of the combinations used are presented in Table 1. The best combination for callus induction from nodal explants was found to be 1.0 mg/L NAA + 1.0 mg/L 2,4-D + 0.5 mg/L BAP (Figure 2). Preliminary results showed that compared to single auxin, a combination of two auxins is more suitable for the callus induction