The primary limitation of this study was the fact that there is no objective evidence to indicate the causal relationship between the identified factors and the occurrence of perceived stresses related to centrifuge training. Future studies could accordingly engage in a series of surveys to further confirm the causal rela-tionship of the identified constructs on subjective stress. This can best be accomplished by comparing the results obtained from physical measures such as heart rate, blood pressure, muscle tension and perspiration to psychological measures. Future research based on this study could also estimate the difficulty of particular stressors and trainees’ ability to adapt to centrifuge training by adopting the polytomous partial credit model of item response theory to regulate efficient training programs for human-use centrifuge with respect to rank and to effectively reinforce trainees’ G tolerance to improve training quality and ensure flight safety.