Quantification of AMF populations in soil and roots has traditionally been performed by microscopic methods such as spore density (Gerdemann and Nicholson, 1963) and root colonization (Phillips and Hayman, 1970 and Giovannetti and Mosse, 1980). These methods rely on the individual scientist's skills in microscopic observations (Gange et al., 1999) and often lack reproducibility amongst different observers.