Quartz crystal microbalance (QCM) sensors coated with molecular imprinted polymers (MIP) have been
developed for the determination of thymine. In this method, methacryloylamidoadenine (MA-Ade) have
used as a new monomer and thymine template for inspiration of DNA nucleobases interaction. The
thymine can be simultaneously hydrogen binding to MA-Ade and fit into the shape-selective cavities. Thus,
the interaction between nucleobases has an effect on the binding ability of the QCM sensors. The binding
affinity of the thymine imprinted sensors has investigated by using the Langmuir isotherm. The thymine
imprinted QCM electrodes have shown homogeneous binding sites for thymine (K
a
:1.0×10
) while
heterogeneous binding sites for uracil. On the other hand, recognition selectivity of the QCM sensor based
on thymine imprinted polymer toward to uracil, ssDNA and ssRNA has been reported in this work.
5
M
−1
© 2009 Elsevier B.V. All rights reserved.