Subsequently, 0.05 g seeds of diploid, triploid and tetraploid of Phal. aphrodite subsp. formosana are processed for surface disinfection by 0.3% sodium hypochloride solution for 5 minutes, and then are washed with sterilized water three times under aseptic condition. The seeds are sown in the MSp medium, and are cultured in a culture room where the temperature is within 25±2□, the illuminance is less than 1,000 1×, and the illuminating time is 16 hrs per day. After culturing for 3 months, protocorms are grown to have a height of 3-4 mm.
Subsequently, 0.05 g seeds of diploid, triploid and tetraploid of Phal. aphrodite subsp. formosana are processed for surface disinfection by 0.3% sodium hypochloride solution for 5 minutes, and then are washed with sterilized water three times under aseptic condition. The seeds are sown in the MSp medium, and are cultured in a culture room where the temperature is within 25±2□, the illuminance is less than 1,000 1×, and the illuminating time is 16 hrs per day. After culturing for 3 months, protocorms are grown to have a height of 3-4 mm.
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