Unlike her real-life counterparts -- almost always young women -- who welcome customers to shops like this, ChihiraAico cannot answer questions, but simply runs through her pre-recorded spiel.
The android, with lifelike skin and almost (but not quite) natural-looking movements, was developed by microwaves-to-power stations conglomerate Toshiba, and unveiled at a tech fair in Japan last year.
"We are aiming to develop a robot that can gradually do what a human does," said Hitoshi Tokuda, chief specialist at Toshiba.
"The standard of customer service in this Mitsukoshi flagship store is top quality and this is a great opportunity to see what role our humanoid can play in this kind of environment."
ChihiraAico will receive customers at the store until Tuesday, before taking part in a series of promotional events over the upcoming Golden Week holidays.
The humanoid is not the first robot to begin customer service in Japan -- the wisecracking Pepper, a four-foot (120 centimetre) machine with a plastic body perched on rollers, flogs coffee machines and mobile phones.