autoclaved sterile cellophane was placed on PDA plate
and a 7mmdiameter disk of the T. harzianum isolate to
be tested was inoculated on the cellophane. The plates
were incubated at 25 ± 18C for 72 h. After the
incubation period, the cellophane was removed and the
center of each dish was inoculated with a disk (7 mm
diameter) of B. oryzae taken from an actively growing
colony. The plates were incubated at 25 ± 18C for a
further 6 days. The control treatment was B. oryzae
grown on PDA only. Seven replicates were used for
each treatment. The percentage of growth inhibition
was calculated using the following equation:
X ¼ ½(G2/G1) 100 100
where X was the percentage of reduction in mycelial
growth, G1 was the averaged growth of pathogenic
fungus in control plates, and G2 was the averaged
growth of pathogenic fungus in treated plates.