To cut sections, the tissue has to be embedded in paraffin wax, but wax is not soluble in water or alcohol.
However, it is soluble in a paraffin solvent called 'xylene'.
Therefore, the water in the tissue needs to be replaced with xylene.
To do this, first the tissue has to be dehydrated, by gradually replacing water in the sample with alcohol.
This is achieved by passing the tissue through increasing concentrations of ethyl alcohol (from 0 to 100%).
Finally, once the water has been replaced by 100% alcohol, the alcohol is replaced with xylene, which is miscible with alcohol.
This final step is called 'clearing'
To cut sections, the tissue has to be embedded in paraffin wax, but wax is not soluble in water or alcohol. However, it is soluble in a paraffin solvent called 'xylene'. Therefore, the water in the tissue needs to be replaced with xylene. To do this, first the tissue has to be dehydrated, by gradually replacing water in the sample with alcohol. This is achieved by passing the tissue through increasing concentrations of ethyl alcohol (from 0 to 100%). Finally, once the water has been replaced by 100% alcohol, the alcohol is replaced with xylene, which is miscible with alcohol. This final step is called 'clearing'
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