In the
final pMH54-gp33/EGFPconstruct, the ATG for ORF4a is 50 nucleotides downstream
of the gene 3/gene4 intergenic sequence. This is followed by the nine
codons of ORF4a, the nine codons of gp33, seven intervening codons followed by
the ATG for the EGFP gene, and the rest of the EGFP gene, such that ORF4a,
gp33, and the EGFP protein are all in frame
In thefinal pMH54-gp33/EGFPconstruct, the ATG for ORF4a is 50 nucleotides downstreamof the gene 3/gene4 intergenic sequence. This is followed by the ninecodons of ORF4a, the nine codons of gp33, seven intervening codons followed bythe ATG for the EGFP gene, and the rest of the EGFP gene, such that ORF4a,gp33, and the EGFP protein are all in frame
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