The mechanisms regulating the expression of maize resistance genes against Aspergillus flavus are poorly
understood. This study examined the potential roles of six WRKY transcription factors and the expression
of three pathway indicator genes in response to A. flavus inoculation in B73 (susceptible) and TZAR101
(resistant). The genes ZmWRKY19, ZmWRKY53, and ZmWRKY67 were found to possess elevated expres-
sion in TZAR101. ZmNPR1 expression was induced by inoculation in TZAR101 without concurrent in-
duction of ZmPR-1, possibly due to the induction of ZmERF1. These findings indicate that WRKY
transcription factors are involved in resistance and that salicylic acid and ethylene signaling may coor-
dinate defense responses.