for the bacterial synthesis of [Met]prochymosin was to reconstruct
a gene in vitro from restriction fragments of a cDNA clone
and then use synthetic DNA fragments for insertion into a bacterial
plasmid immediately downstream from the strong E. coli
trp promoter, a functional ribosome binding site and ATG. As
shown in Fig. 1A, a BamHI restriction site is conveniently located
between codons 5 and 6 of the prochymosin sequence.