The RS contents of acid and enzyme dextrinized retrograded starches were presented in Table
2. The starch prepared by dextrinization with pullulanase enzyme and retrogradation gave the RS
content much higher than the sample obtained by acid dextrinization and retrogradation. The RS
contents of corn and cassava starches increased from 9.06 to 22.49% and 6.33 to 28.31%,
respectively. Corn and cassava starches contain the branch amylopectin molecules which prevents
starch retrogradation. Pullulanase, a debranching enzyme has the ability to specifically hydrolyze at a
branch point or debranch gelatinized starch. Zhao and Lin (2009) had applied hydrochloric acid, citric
acid, acetic acid and pullulanase to debranch gelatinized maize starch and found that the debranching
effect of pullulanase on maize starch was more effective than acid hydrolysis method. Pullulanase is
frequently used to hydrolyze α - 1,6 linkage in amylopectin to eliminate amorphous regions, thus short
linear segments of α - 1, 4 glucan are arranged in the crystalline structure (Guraya et al., 2001)