Fig. 5. Model showing MurG localization and function. (A) Cells show three different stages of B. subtilis sporulation engulfment during sporulation. In red are shown zones of extreme negative curvature that are accessible from the mother cell cytoplasm and presumably preferred by cardiolipin. (B) In the presence of cardiolipin, MurG is localized to membranes of high negative curvature via an amphipathic helix. Residues V74 and F77 within this helix are required for localization (yellow). (C) Synthesis of Lipid II by MurG is regulated by its localization to the cell membrane during sporulation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)