In the present study, we used proteomics to investigate the physiology and molecular phenotype of peanut and maize under monocropping and intercropping, based on 2-DE and MALDI MS/MS approaches. The combination of 2-DE-based protein separation with tandemmass spectrometry for peptide sequencing is an attractive method for the characterization of organisms for which the genome sequence is not yet available.
We utilized a variety of physiological, transcriptome and proteome techniques to evaluate the responses of peanuts andmaize to intercropping and monocropping conditions in an
environmentally controlled greenhouse. The overall objective of this research was to develop a protein reference map of the ecological significance of peanut/maize intercropping