Production of biomass
For the production of biomass, the actinomycetes strains such as Streptomyces sp. I(P1), Rhodococcus sp. (P8) and Streptomyces sp. II(NP1) were grown aerobically in actinomycetes broth. The culture flasks were incubated on room temperature at 37oC. After 15 days of incubation period, biomass was harvested using Whatman filter paper. The harvested biomass was washed with double distilled water and the biomass was filtered. The filtered biomass was immersed in double distilled water for 24 hours. After incubation, the biomass was separated from the layer of water (cell free filtrate) for further studies.