Sugarcane juice was boiled for 10 min, cooled to room
temperature, and the dextrans allowed to settle. The supernatant
(clarified juice) for the ethanol fermentation was
placed in 2 L flasks with 1.25 L juice per flask at 14°B.
The fermentation medium was inoculated with an overnight
culture (grown for ~16–20 h) of S. cerevisiae G at
106 cell mL–1. Samples were withdrawn every 12 h and °B
determined using a hand refractometer. Ethanol was determined
by the method of Caputi et al.4 The fermented
sugar cane was allowed to settle for 2 days at 4°C and the
supernatant was siphoned off. The ethanol content was
adjusted to 7% (v/v) using distilled water and was inoculated
with the adsorbed/entrapped cells of A. aceti cells.