The following microbiological media were used:
– Y east Extract Agar (Merck) to determine the total
number of bacteria;
– Sabouraud Agar medium with 2% of glucose (Merck)
for quantification of fungi;
– Czapek-Doxa Agar (BTL) for filamentous fungi identification.
Petri dishes were incubated for 24–48 hours at 37oC
(to determine the total number of bacteria) and for 10
days at 25oC (to enumerate fungi). Results were shown by
colony forming units in 1 m3 of air (cfu/m3).
Bacteria were identified by three arrays. The first one
– macroscopic estimation (description of colony). The
second one – microscopic estimation (dyeing by Gram
and Schaeffer-Fulton method). The third one – biochemical
tests according to bacteria classification according to
Bergey [34].
Diagnosis of filamentous fungi was based on estimation
of morphological features of growth on Czapek medium as well as on microscopic observation according to
filamentous fungi estimation guide [35].