which are fundamental in the expression
of diverse genes, could generate diametrically opposed conclusions
given their rapid expression levels,low expression levels,and high transcript instability (data not shown, manuscript in preparation).
In conclusion, the present study provided evidence that the reference genes β-actin, CD81, and YWHAH could be useful for transcript
quantification under several MDBK culture conditions. Importantly, incorporating multiple reference genes provides the most reliable expression pattern after precise normalization.