Genotyping of SNP markers
was performed using DNA MassArray technology ( Jurinke
et al. 2002). External and extension primers were designed
using Sequanom’s assay-design software (Table 2). This software
also selected the appropriate termination mix for each
of the SNPs. MALDI–TOF mass spectrometry analysis was
performed using the MassArray genotype analyzer.
Gene mapping: Genotype data for the 11 genes in the F2
mapping population were added to the published data set of
545 markers, and mapping was performed using JoinMap
software (3.0) as previously described (Lee et al. 2005).