2.4.4. Ferrous reducing power
The reducing power was determined as described in our previous
report (Kuda & Yano, 2009) with slight modification. Briefly,
each 0.05 mL of the sample solution, 0.025 mL of 0.1 mol/l phosphate
buffer (pH 7.2), and 0.025 mL of 10 g/l potassium ferricyanide
were placed in a 96-well microplate. After incubation at
37 C for 60 min, 0.025 mL of 10% TCA and 0.1 mL of distilled water
were added and the absorbance was measured at 700 nm (Abs1).
Next, 0.025 mL of 0.1% FeCl3 was added to the mixture and the
absorbance was measured again (Abs2). Ferrous reducing power
was calculated using the following formula:
Reducing power ðOD 700nmÞ¼ðAbs2 of sampleAbs1 of sampleÞ
ðAbs2 of controlAbs1 of controlÞ
2.4.4. Ferrous reducing powerThe reducing power was determined as described in our previousreport (Kuda & Yano, 2009) with slight modification. Briefly,each 0.05 mL of the sample solution, 0.025 mL of 0.1 mol/l phosphatebuffer (pH 7.2), and 0.025 mL of 10 g/l potassium ferricyanidewere placed in a 96-well microplate. After incubation at37 C for 60 min, 0.025 mL of 10% TCA and 0.1 mL of distilled waterwere added and the absorbance was measured at 700 nm (Abs1).Next, 0.025 mL of 0.1% FeCl3 was added to the mixture and theabsorbance was measured again (Abs2). Ferrous reducing powerwas calculated using the following formula:Reducing power ðOD 700nmÞ¼ðAbs2 of sampleAbs1 of sampleÞðAbs2 of controlAbs1 of controlÞ
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