a b s t r a c t
Pumpkin seeds have been implicated in providing health benefits. However their antioxidant or antiinflammatory
activity of their extracts has never been studied. Therefore, four commercially available
pumpkin seeds were treated with two different extraction methodologies in order to obtain fractions
with different content. The extracts were screened for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl
(DPPH) free radical scavenging assay and for their inhibitory activity against lipid peroxidation
catalyzed by soybean lipoxygenase.
Most extracts tested have demonstrated radical scavenging activity, which depends on their total phenolic
content, with fractions rich in phenolics showing the strongest activity. On the other hand, the phenolic
content of extracts does not determine their activity against lipoxygenase, as acetone and polar lipid
fractions are its strongest inhibitors. The presence of molecules being able to scavenge radicals and inhibit
lipoxygenase in pumpkin seeds may in part explain the health benefits attributed to them.
a b s t r a c tPumpkin seeds have been implicated in providing health benefits. However their antioxidant or antiinflammatoryactivity of their extracts has never been studied. Therefore, four commercially availablepumpkin seeds were treated with two different extraction methodologies in order to obtain fractionswith different content. The extracts were screened for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging assay and for their inhibitory activity against lipid peroxidationcatalyzed by soybean lipoxygenase.Most extracts tested have demonstrated radical scavenging activity, which depends on their total phenoliccontent, with fractions rich in phenolics showing the strongest activity. On the other hand, the phenoliccontent of extracts does not determine their activity against lipoxygenase, as acetone and polar lipidfractions are its strongest inhibitors. The presence of molecules being able to scavenge radicals and inhibitlipoxygenase in pumpkin seeds may in part explain the health benefits attributed to them.
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