The oyster mushroom grown on substrates R80 + WB20 and WS80 + WB20 gave the significantly longer stipe length than that on substrate C80 + WB20. The oyster mushroom grown on substrate C45 + R45 + WB10 gave significantly shorter stipe length than that on substrate R80 + WB20, and grown on substrate C40 + WS40 + WB20 had significantly shorter stipe length than that on substrate WS80 + WB20. When compared to non-sterilized substrate, the oyster mushroom on sterilized substrates C80 + WB20, C45 + R45 + WB10, C30 + R60 + WB10, C40 + WS40 + WB20, C30 + WS50 + WB20 and C20 + WS60 + WB20 has significantly shorter stipe length.