Microbiological techniques
ISO 21528-2:2004
1.0 mL aliquots of each of the dilutions were transferred to Petri plates and added with about previously melted 10 mL of the Violet Red Bile Glucose agar culture medium (VRBG – Oxoid Ltd., Basingstoke, Hampshire, England), and kept at 44 °C – 47 °C in a water bath. The inoculum was carefully homogenized with the culture medium until total solidification.