2.2. Culture establishment
Single-nodes, 1.5–2 cm long were prewashed in a commercial dish washer (Rika)
solution (about 0.1%) for 15 min and then placed under running tap water until used. The
explants were surface sterilised by immersion in 10% chlorox (5.25% NaOCl) for 15 min
and then rinsed 3 times in autoclaved distilled water.