This was deemed time zero for incubation and was not interrupted until all the G120 portions were collected (see
below). The enzyme mixture was added to each flask at 1-min intervals to aid timing of incubations. After 20 min, an aliquot
(0.5 ml, G20) from each flask was taken into a centrifuge tube containing 20 ml of ethanol (66 g/L). After 100 min, a second 0.5 ml sample (G120)was taken in the sameway. The G20 and G120 portions were centrifuged at low speed (1500g) for 5 min.