In the edible mushroom cultivation industry, usually grain spawn is used. However, it has been reported that preparation of grain spawn has longer growth period and poses higher risk of contamination compared to liquid spawn (Confortin et al., 2008). Mycelia or liquid spawn produced by submerged fermentation is an alternative method for generating spawn as it produces higher yield and more uniform mycelium biomass in a shorter period (Stamets, 2000), favours mycelium dispersion, adaptation and enables inoculation to be carried out under a relatively more stringent aseptic condition (Bettin et al., 2009).