Fatty acids in the methanol extracts from the oils were
then analyzed by using high performance liquid chromatography (HPLC) (Shimadzu, LC-10AT) which consisted
of the column (Capcell Pak C18, 25 cm in length · 4.6 mm
in inner diameter, 5 lm, Shiseido Finechemicals. Co.) and
ultraviolet detector at 220 nm (Tosoh, UV-8024) operated
at 30 C with 0.7 ml/min flow rate of 80% acetonitrile solution containing 20% of 0.1% H3PO4 as a carrier solvent.
The sample volume was 20 ll and a peak identification
was made by comparing the reaction time between the sample and the standard compound