INTRODUCTION
Mastitis causes physical, chemical and bacteriological alternation in the milk and blood along with morpho-patholological changes in the mammary gland. It causes a great economic loss in the dairy industry, world-wide. Subclinical mastitis (SCM) is a herd problem and frequently overlooked, causing additional economic loss (Gera et al., 2006). SCM detection is precluded due to pooling of milk for sale from the village or the farm and as a result of which the source of the SCM milk cannot be determined after collection (Gera and Guha, 2011a). For years many workers tried to develop several indirect tests, but, unfortunately none proved satisfactory to diagnose SCM