2.5.3. Extraction, identification and quantification of acrylamide The extraction was performed with the technique proposed by Biedermann et al. (2002). From the extract obtained, 2.0 ml of each sample were injected in duplicate.
A gas chromatograph (trade-mark Hewlett-Packard, model 1800B, GCD System, Santa Clara, CA,USA), equipped with a column CP-WAX 52CB (VARIAN)
(60 m 0.25 mm 0.25 mm), was used for the analysis.
The initial temperature of 80 C was maintained for 5 min and subsequently