Downstream processing of the bioproducts obtained is one of the
challenges ahead of any co-production system. While classical
downstream methodologies based on precipitation and further
crystallization from the clarified broth are well established
(Alonso et al., 2013a), ion exchange methodologies have recently
come to the fore when binary or ternary mixtures from a production
process like the one studied herein are achieved (Borges da Silva
et al., 2011; Pedruzzi et al., 2008). Their high selectivity and process
yields make ion exchange-based methodologies well-suited for lactobionic
acid purification. However, further improvements on
bio-based lactobionic acid purification are necessary to approach
an effective integrated bio-production system with further downstream
separation from a complex matrix like cheese whey.
Overall, the mixed carbon source strategies developed in the present
study demonstrate the potential for large-scale bio-production of
lactobionic acid through a dairy biorefinery strategy. The whey/glucose
co-fermentation developed herein constitutes a technology
platform to co-produce lactobionic and gluconic acid into an integrated
single-cell biorefinery. Such dairy biorefinery approach will
allow dairy manufacturers to combine cheese whey and dairy
glucose-based waste streams, yielding a better product portfolio
with interests in cosmetics and personal care products