The separation was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v/v) on Phenomenex C18 column (5.0 lm, 4.6 150 mm),
The separation was done with isocratic elution of amobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v/v) on Phenomenex C18column (5.0 lm, 4.6 150 mm),