2.8. Metabolism of glucose, sucrose and mannitol
Stems below the position where first floret bud was generated were cut into disks with length of 5 mm. Disks (approximately 500 mg) were placed into a solution containing 250 mM 14C-glucose, 14C-sucrose or 14C-mannitol solution (61.79 kBq, 37 MBq mmol1) in a Petri dish and kept at 23 C under darkness for 1 h. The disks were then transferred into 250 mM unlabeled glucose, sucrose, and mannitol solutions for 0, 6, 12 and 24 h and then immersed in 80% ethanol. The discs were homogenized in 80% ethanol and centrifuged. The resulting supernatant was combined and evaporated to dryness in vacuo below 50 C. The residue was dissolved in water and separated by HPLC equipped with a radioanalyzer (RLC-113, Aloka, Tokyo, Japan). The column and separation conditions were as described above.
Fig. 2. Spike length (A), bud number (B) and open flower number (C) of cut snapdragons treated with 250 mM glucose, sucrose, mannitol and sorbitol. All solutions including the control contained 200 mg L1 8-HQS and cut flowers were held at 23 C. The number of flower buds was the sum of the flower buds longer than 5 mm, open flowers, and wilted flowers. Values are the mean of 8 replicates SE (A and B) or +SE (C).
Fig. 3. Photographs of cut snapdragon flowers treated with various carbohydrates at 250 mM. All solutions including the control contained 200 mg L1 8-HQS. Cut flower spikes treated with 250 mM glucose, sucrose, sorbitol, or mannitol at 23 C for 14 days (A). Far left, control; middle left, glucose; middle, sucrose; middle right, sorbitol; far right, mannitol. Cut flower spikes treated with 250 mM mannitol at 23 C for 25 days (B). Upper part of cut flower spikes treated with 250 mM mannitol at 23 C for 27 days (C).
2.8. Metabolism of glucose, sucrose and mannitolStems below the position where first floret bud was generated were cut into disks with length of 5 mm. Disks (approximately 500 mg) were placed into a solution containing 250 mM 14C-glucose, 14C-sucrose or 14C-mannitol solution (61.79 kBq, 37 MBq mmol1) in a Petri dish and kept at 23 C under darkness for 1 h. The disks were then transferred into 250 mM unlabeled glucose, sucrose, and mannitol solutions for 0, 6, 12 and 24 h and then immersed in 80% ethanol. The discs were homogenized in 80% ethanol and centrifuged. The resulting supernatant was combined and evaporated to dryness in vacuo below 50 C. The residue was dissolved in water and separated by HPLC equipped with a radioanalyzer (RLC-113, Aloka, Tokyo, Japan). The column and separation conditions were as described above.Fig. 2. Spike length (A), bud number (B) and open flower number (C) of cut snapdragons treated with 250 mM glucose, sucrose, mannitol and sorbitol. All solutions including the control contained 200 mg L1 8-HQS and cut flowers were held at 23 C. The number of flower buds was the sum of the flower buds longer than 5 mm, open flowers, and wilted flowers. Values are the mean of 8 replicates SE (A and B) or +SE (C).Fig. 3. Photographs of cut snapdragon flowers treated with various carbohydrates at 250 mM. All solutions including the control contained 200 mg L1 8-HQS. Cut flower spikes treated with 250 mM glucose, sucrose, sorbitol, or mannitol at 23 C for 14 days (A). Far left, control; middle left, glucose; middle, sucrose; middle right, sorbitol; far right, mannitol. Cut flower spikes treated with 250 mM mannitol at 23 C for 25 days (B). Upper part of cut flower spikes treated with 250 mM mannitol at 23 C for 27 days (C).
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