Acid Hydrolysis and Solution Making with H2,so4, Hydrolysis is necessary to convert starches to fermentable sugars. The cell degradation of carbohydrate to fermentable sugar basically done with the hydrolysis process by making solution with Acid.The term"Acid Hydrolysis" is also applied to certain nucleophilic addition reactions, such as in the acid catalyzed hydrolysis of nitriles to amides. hydrolysis usually refer to the acid catalyzed addition of the elements of water to double or triple bonds by electrophilic addition as may originate from a hydration reaction. For our work purpose we have made three sample solutions with the variation of Sulphuric acid percentage. Every sample solution is 500 ml solution. This solution was made with algal powder and distilled water. Three samples were made with 1%, 1.5% & 2% sulphuric acid by weight percentage. Steam Autoclaving the Solution An autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 120-130oC for around 15-30 minutes depending on the size of the load and the contents. In our process at this step autoclaving is required for both the perfect cell degradation that means carbohydrate cell coming out form the spirulina cell and for assisting the acid hydrolysis process. We complete the process in the laboratory of Department of Microbiology, University of Dhaka. Three samples are made according to the autoclaving time 15 min, 20 min, and 25 min at 1250 C. The pressure of the autoclave was 15 psi