Phosphate is one of the important macronutrients for microalgae
that are presented in lipids and nucleic acids and also act as
intermediates for carbon metabolism [6]. Also, the metabolic
uptake of phosphate begins with phosphorylation which converts
ADP to ATP [24]. The removal of phosphate by C.
pyrenoidosa and S. abundans is presented in Fig. 4a, Fig. 4b
respectively. It can be noted from Fig. 4(a) that the maximum
phosphate removal by C. pyrenoidosa cultivated in raw sample
with the inoculum size of 10% and 20% was observed as 97%
and 96%, respectively, whereas, in autoclaved sample with the
inoculum size of 10%, it was observed as 86%. Similarly, it can
be noted from Fig. 4(b) that the maximum phosphate removal
by S. abundans cultivated in raw sample with the inoculum size
of 10% and 20% was observed as 96% and 97%, respectively,
whereas, in autoclaved sample with the inoculum size of 10%,
it was observed as 96%. Notably, C. pyrenoidosa with inoculum
size of 10% showed variation compared to 20% inoculum
size in raw sample which is due to prolonged acclimatization
period.