4. At 30ºC, the semen is diluted to 50% of the final
volume of diluents using the 3% glycerol diluents half of the
final volume without any glycerol. Semen is examined
under a microscope for individual motility. Semen is
transferred into the cold cabinet at +5ºC and allowed to cool
and stabilize for about 30 min. The second part 11%
glycerol diluent or half of the final volume with 14 mL
(14%) glycerol content is added to the semen to make up the
total volume which will yield 7% glycerol content.