reduction after treatment at 25 °C. Considering that heat treatment alone reduced this pathogen by about 2.16 log at 50 °C, a minimum of 3.66 log reduction could be expected when both ozone and heat treatments are performed at 50 °C. However, approximately 4.85 log reduction was obtained. Similarly, the combination treatment resulted in an enhanced reduction ofS.Typhimurium andL. monocytogenes(about 5.30 and 3.79 log CFU/ml), which is greater than the sum of the reductions (about 4.34 and 2.05 log CFU/ml, respectively) obtained through single treatments of ozone or 50 °C. This indicates that the combination of ozone and heat treatments at 50 °C can produce a synergistic effect in pathogen inactivation of apple juice.