Twenty milliliters of mango nectars was mixed with 100mL of 2.5%
metaphosphoric acid, incubated at 4 °C for 2 h. Thereafter, the mixture
was centrifuged using 9000 ×g for 15 min at 4 °C, and the supernatant
was subsequently used for HPLC measurement. The HPLC system
(RF-10AXL, Shimadzu Co., Japan) was equipped with a prominence
UV–visible detector (SPD-20AV), a system controller (CBM-20A), an
auto sampler (SIL-20A), two pumps (LC-20AT), and a column oven
(CTO-20A). The analytical column was a Sunfire™ C18 (4.6 × 250mm
Twenty milliliters of mango nectars was mixed with 100mL of 2.5%metaphosphoric acid, incubated at 4 °C for 2 h. Thereafter, the mixturewas centrifuged using 9000 ×g for 15 min at 4 °C, and the supernatantwas subsequently used for HPLC measurement. The HPLC system(RF-10AXL, Shimadzu Co., Japan) was equipped with a prominenceUV–visible detector (SPD-20AV), a system controller (CBM-20A), anauto sampler (SIL-20A), two pumps (LC-20AT), and a column oven(CTO-20A). The analytical column was a Sunfire™ C18 (4.6 × 250mm
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